Continuous cropping obstacles in orchards affect soil nutrient mineralization and availability，and are closely related to the growth and development of seedlings. To investigate the effects of continuous cropping barriers on soil microbial communities and metabolites in peach multi-planting seedling sites，natural grassland was used as the control(CK，uncultivated grass)and peach seedling land with 3 years of continuous cropping as the research object(0-20，20-40 cm)，and Illumina high-throughput sequencing technology combined with LC-MS non-targeted metabolomics analysis were used to study the characteristics of the changes in soil microbial community structure and metabolites of the peach seedling land in the process of continuous cropping. Compared with the CK treatment，the content of organic matter，available potassium，available iron and available zinc decreased，and the content of total nitrogen and total phosphorus increased in the soil with 3 years of continuous cropping. The diversity of the bacterial community was significantly reduced，and the key microorganisms constituting the molecular ecological network of the bacteria were reduced，which led to a decrease in the stability of the bacterial community. The metabolomics analyses revealed a significant increase in the content of acids in the soil with continuous cropping，and the content of 4-(benzyloxy)benzoic acid，(R)-3-hydroxymyristic acid，3，5-dimethoxybenzoic acid and other potential autotoxic substances accumulated significantly. Continuous cropping had significant effects on arachidonic acid metabolism，serotonin synapses and antibiotic biosynthetic pathways in soil. Correlation analyses revealed that soil pH，and available iron，available zinc，total salt，total nitrogen，alkaline hydrolysis nitrogen，organic matter，available potassium contents were the main factors affecting the changes in soil bacterial communities in peach multi-planting seedling sites，whereas palmitic acid，5-chloro-2，8-dimethyl-4-[(3-nitro-2-pyridinyl)oxy] quinoline and γ-nonanolactone were the main metabolites regulating the soil bacterial communities in peach multi-planting seedling sites. In summary，compared with the same layer of CK，organic matter，total phosphorus，available potassium，available iron and available zinc contents were decreased more in the 20-40 cm soil layer than those in the 0-20 cm soil layer. Changes in the dominant bacterial phyla and genera resulted in a significant increase in the content of acid substances in soil，biosynthetic pathways of arachidonic acid metabolism，serotonin synapses and antibiotics were enriched with higher intensity and the content of differential metabolites was significantly reduced. The correlation analysis showed that，changes in soil microbial communities in peach multi-planting sites were significantly correlated with soil pH，available iron and available zinc contents，while metabolites such as palmitic acid，5-chloro-2，8-dimethyl-4-[(3-nitro-2-pyridinyl)oxy]quinoline，and γ-nonanolactone were the main metabolites affecting the structure of soil bacterial communities in peach multi-planting seedling sites.