The effects of nitrogen-potassium ratio on nutrient accumulation，yield，and quality of flue-cured tobacco were analyzed，and the most suitable nitrogen-potassium ratio was clarified in Liangshan area，which provided a theoretical basis for optimizing the formula of special fertilizer for flue-cured tobacco. Field experiments with different nitrogen-potassium ratios were carried out in Liangshan Huili and Yuexi tobacco areas，and four different nitrogen-potassium ratios were set up，which were 1∶1.5(K1.5)，1∶2(K2)，1∶2.5(K2.5)and 1∶3(K3)，respectively，to analyze the changes of dry matter accumulation and nutrient accumulation in each organ of flue-cured tobacco. The experiment indicated that different nitrogen-potassium ratios had a significant impact on the growth，development，and nutrient accumulation of flue-cured tobacco. Appropriately reducing the nitrogen-potassium ratio significantly increased the dry matter accumulation in tobacco leaves. Compared to K1.5，the dry matter accumulation in leaves treated with K2.5 in Huili and Yuexi increased by 11% and 15%，respectively，enhancing the tobacco’s ability to absorb nitrogen，phosphorus，and potassium. However，too low a nitrogen-potassium ratio had no significant effect on the growth and nutrient accumulation of flue-cured tobacco. Compared with K1.5，increasing the application of potassium fertilizer significantly improved the nitrogen，phosphorus，and potassium content in various organs of flue-cured tobacco. In the Huili area，the leaf nitrogen content，leaf phosphorus content，and leaf potassium content of the K3 treatment increased by 35%，68% and 5%，respectively，compared to the K1.5 treatment. There was no significant difference in nutrient accumulation between the K2.5 and K3 treatments in the Huili area，while the K2.5 treatment performed better in the Yuexi area. Based on the results from both locations，a nitrogen-potassium ratio of 1∶2.5 could be considered the most suitable fertilization scheme for the Liangshan area.