This study aimed to investigate the impact of inoculation sequences from three microbial groups on the humus composition associated with different mineral types involved in lignin-mineral complex formation.The liquid shaking bottle method was adopted to inoculate six distinct microbial sequences(ABS，ASB，BAS，BSA，SBA，and SAB)，which comprised three strains:Bacillus megaterium(B)，Streptomyces griseus(S)，and Aspergillus niger(A)within a lignin culture medium containing goethite(Goe)，bayerite(Bay)，δ-MnO₂(MnO)，kaolinite(Kao)，and montmorillonite(Mon).This approach aimed to elucidate the variations in the composition of lignin-mineral complexes formed during humification，and explored how different bacterial，actinomycete，and fungal inoculation sequences influenced the lignin humification process.The results indicated that:(1)The incorporation of kaolinite in various inoculation sequences enhanced thealiphatic structure of HLA molecules post-culture，leading to a reduction in molecular condensation，with the sequence A.niger→B.megaterium→S.griseus exhibiting the most pronounced effect；Similarly，montmorillonite addition demonstrated an analogous trend.(2)Goethite，δ-MnO₂，and bayerite exhibited superior promoting effects on HLA formation compared to kaolinite and montmorillonite，with goethite yielding the most significant results.When these five minerals wereadded，HLA molecules showeda greater tendency for degradation under the inoculation sequence B.megaterium→A.niger→S.griseus；conversely，the sequence A.niger→B.megaterium→S.griseus combined with either goethite or δ-MnO₂ was more conducive to enhancing CHLA levels.(3)Under identical conditions，goethite significantly outperformed other minerals in improving humus quality；Specifically，under δ-MnO₂ supplementation，only the B.megaterium→A.niger→S.griseus sequence resulted inacontinuous decline in the C content ratio(CHLA/CFLA)between humic acid and fulvic acid(FLA)after 110 days of culture，whereas all other inoculation sequences led to varying degrees of increase in CHLA/CFLA ratios when bayerite or kaolinite were added alongside S.griseus→A.niger→B.megaterium sequencing which notably raised CHLA/CFLA levels，and the largest increment was observed with S.griseus→B.megaterium→A.niger sequencing.(4)Identical inoculation sequences paired with different mineral types influenced both abundance and deficiency of CHLu after cultivation，thus appropriate combinations of inoculation sequences and mineral types facilitated humin(HLu)decomposition.For instance:under kaolinite conditions using S.griseus→A.niger→B.megateriumasan inoculation sequence or employing goethite along with A.niger→S.griseus→B.megaterium or δ-MnO₂ combined with A.niger→B.megaterium→S.griseus effectively promoted HLu degradation processes. Goethite proved optimal for enhancing humic quality while fostering HLA formation was more likely through culturing via A.niger→B.megaterium→S.griseus. Additionally，A.niger→S.griseus→B.megaterium supplemented by goethite appeared particularly effective at facilitating inert humic component(HLu)decomposition into other active humic component.