This study was aimed to reduce the use of chemical fertilizers and screen suitable fertilizer combinations for high-quality and high-yield greenhouse tomatoes in Yuzhong，Gansu. Nine treatments were set up，including no fertilization(NF)，local chemical fertilizer+planting and breeding waste compost 0(C0)，6000(C4)，9000(C6)，12000(C8)kg·hm-2，and local chemical fertilizer reduction by 30%+planting and breeding waste compost 0(R0)，6000(R4)，9000(R6)，and 12000(R8)kg·hm-2. Effects of fertilizer reduction and composting of planting and breeding waste on yield，quality and nutrient absorption of greenhouse tomatoes were studied. The results showed that R6 was the best treatment，which not only significantly increased the yield and quality，but also effectively increased the absorption of nitrogen，phosphorus and potassium nutrients. Compared with R0 treatment，the yield of R6 treatment increased by 15.33%，the contents of vitamin C，soluble sugar，soluble solids and soluble protein in fruit increased by 8.75%，6.57%，7.42% and 14.29%，respectively. Moreover，organic acid content decreased by 4.14%. The change of mineral element content in each treatment was not obvious. Contents of sucrose was significantly lower than that of fructose and glucose. Oxalic acid content was the lowest in R6 treatment，while the contents of other acids under eight fertilizer treatments was higher than those under NF treatment. Compared with NF treatment，the accumulation of nitrogen，phosphorus and potassium in R6 treatment increased by 94.71%，80.95% and 89.09%，and the utilization rate increased by 68.48%，141.85% and 104.11%，respectively. In addition，principal component analysis showed that the comprehensive score of R6 treatment was the highest. Therefore，the fertilization combination mode of R6 could be used as the best fertilization scheme in Yuzhong area and could be promoted in the surrounding areas.