In order to explore the resource utilization of duck waste liquid，the effects of glucose addition，solid-liquid ratio and incubation time on post-fermentation biomass were studied，and the optimal fermentation conditions were determined by single factor test and response surface design. The results showed that Saccharomyces cerevisiae W-1 was used as the fermentation strain at 30 ℃ and 130 r/min，and the optimal fermentation conditions were 5.54%，1.5 and 72 hours for glucose addition，duck waste liquid ratio and incubation time，respectively. Under these conditions，the removal rate of chemical oxygen demand reached 82.84% and the increase of total nitrogen reached 57.04% after fermentation of duck waste liquid. After 15 days of treatment with fermentation broth，the rice seedlings could grow normally at different concentration levels，and the rice indexes in the treatment group were significantly higher than those in the clear water control group，and showed a trend of first increasing and then decreasing，with the maximum value at the dilution factor of 60 times，and there were significant differences in plant height，total root length，root surface area，root tip number，total root volume，dry weight and chlorophyll content from other treatment groups. In summary，the fermentation conditions for efficient duck waste treatment were obtained，and compared with the unfermented duck waste liquid，the fermentation broth had a certain growth-promoting effect on the growth of rice.