香菜促生型生物有机肥的筛选与评价
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(云南农业大学资源与环境学院,云南 昆明 650201)

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李勋(1998-),硕士研究生,从事农业固体废物肥料化利用的研究。E-mail:443369867@qq.com。

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基金项目:云南省重大科技专项(202302AE090012,202202AE090025);国家自然科学基金(32160744);云南省高层次人才培养计划青年人才专项(YNWR-QNBJ-2019-249)。


Screening and evaluation of coriander growth-promoting bio-organic fertilizer
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(College of Resources and Environment,Yunnan Agricultural University,Kunming Yunnan 650201)

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    摘要:

    为筛选出对香菜具有高效促生能力的生物有机肥。以香菜为研究对象,在等养分条件下设置化肥(CK)、有机肥(OF)、枯草芽孢杆菌生物有机肥(BF1)、酿酒酵母生物有机肥(BF2)、毕赤酵母生物有机肥(BF3)5个处理进行盆栽试验。通过研究不同施肥处理对香菜的生长指标、土壤理化性质及酶活性的影响,结合主成分分析进行评价,筛选出促生效果最优的生物有机肥。与CK相比,BF1、BF2和BF3处理的香菜生物量、地上部鲜重及地下部鲜重分别显著增加13.12%~35.95%、16.36%~41.8%及4.74%~20.86%,其中以BF3处理的生物量最高;BF1、BF2和BF3处理显著提高香菜根系活力、根长、根体积、根表面积、根尖数、根直径及植株养分含量,有效促进香菜根系的生长分化及养分吸收。BF1、BF2和BF3处理均改善土壤理化性质及酶活性,土壤电导率降低19.43%~24.02%、有机质增加44.20%~66.18%、碱解氮增加17.12%~28.17%、有效磷增加30.40%~48.83%、速效钾增加4.49%~9.27%,土壤脲酶活性提高2.8%~5.82%、土壤酸性磷酸酶活性提高11.90%~30.25%、土壤蔗糖酶活性提高21.71%~28.45%。冗余分析表明香菜根尖数和根表面积的改变是影响其生长及养分吸收的主要原因;通过对香菜生长特性的主成分分析,得出BF3处理的促生效果最好。BF1、BF2和BF3处理能够改善土壤肥力及酶活性,提高香菜根系活力、改善根系形态、促进香菜生长;其中以BF3处理的促生效果最好,具有开发为优质促生型生物有机肥的潜力。

    Abstract:

    In order to screen out the bio-organic fertilizer with high growth-promoting ability for coriander,taking coriander as the research object,five treatments of chemical fertilizer(CK),organic fertilizer(OF),Bacillus subtilis bio-organic fertilizer(BF1),Saccharomyces cerevisiae bio-organic fertilizer(BF2),Pichia pastoris bio-organic fertilizer(BF3) were set up under equal nutrient conditions for pot experiment. By studying the growth index of coriander,soil physical and chemical properties and enzyme activity,the bio-organic fertilizer with the best growth-promoting effect was selected. Compared with CK,the biomass,shoot fresh weight and root fresh weight of coriander treated with BF1,BF2 and BF3 increased significantly by 13.12%–35.95%,16.36%–41.8% and 4.74%–20.86%,respectively,and the biomass of BF3 treatment was the highest. The treatments of BF1,BF2 and BF3 significantly increased the root activity,root length,root volume,root surface area,root tip number,root diameter and plant nutrient content of coriander,and effectively promoted the growth and differentiation of root system and nutrient absorption of coriander. The BF1,BF2 and BF3 treatments improved soil physical and chemical properties and enzyme activities. Soil electrical conductivity decreased by 19.43%–24.02%,organic matter increased by 44.20%–66.18%,alkali-hydrolysable nitrogen increased by 17.12%–28.17%,available phosphorus increased by 30.40%–48.83%,available potassium increased by 4.49%–9.27%,soil urease activity increased by 2.80%–5.82%,soil acid phosphatase activity increased by 11.90%–30.25%,and soil sucrase activity increased by 21.71%–28.45%. Redundancy analysis showed that the changes of root tip number and root surface area were the main reasons affecting the growth and nutrient absorption of coriander. Through the principal component analysis of the growth characteristics of coriander,it was concluded that BF3 treatment had the best growth-promoting effect. The treatments of BF1,BF2 and BF3 could improve soil fertility and enzyme activity,increase root activity,improve root morphology and promote growth of coriander. Among them,BF3 treatment had the best growth-promoting effect and had the potential to develop into a high-quality growth-promoting bio-organic fertilizer.

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李勋,王宇蕴,李兰,窦梦玲,高江飞,徐智.香菜促生型生物有机肥的筛选与评价[J].中国土壤与肥料,2025,(4):247-257.

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  • 收稿日期:2024-08-13
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  • 录用日期:2024-10-14
  • 在线发布日期: 2025-06-25
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