To achieve the resource utilization of livestock and poultry manure and improve the soil quality of tea gardens， the effects of swine manure biochar and biogas slurry application on soil humus components and enzyme activities in tea plantations were investigated in a 2-year pot experiment. In the experiment，no swine manure biochar was applied as a control（CK），and swine manure biochar treatments with 1% and 2% swine manure biochar application at 350℃ and 500℃ and 2% swine manure biochar application at 650℃ and swine manure biochar with biogas slurry were set up to analyze the effects of swine manure biochar and biogas slurry application on the soil humus components，the structural characteristics of humic acid，and the enzyme activities of the soil in the tea plantation. The results showed that after two years of swine manure biochar application to the soil，compared with CK，soil organic carbon（SOC）content in 2% 500℃ swine manure biochar（M₂）treatment increased significantly by 4.48 g/kg；Water soluble substance（WSS）contents of the treatments were not significant；Humic acid（HA）and fulvic acid（FA）content in 2% 650℃ swine manure biochar（H₂） treatment significantly increased by 0.52 g/kg and 85.0%，respectively；Humin（HM）content in M₂ treatment increased by 90.2%，and it was significantly higher than other swine manure biochar treatments. PQ values of the treatments were not significantly different for application of swine manure biochar. Soil laccase（SL）activity was significantly decreased by 30.8% in M₂ treatment，and M₂ and H₂ treatments significantly increased soil peroxidase（SPOD）activity by 30.5%， 28.4%，respectively. Soil polyphenol oxidase（SPPO）activity did not differ significantly among treatments. In the combined application of swine manure biochar and biogas slurry treatments，compared with the control，SOC content was significantly increased by 4.90 g/kg in combined application of 2% 500 ℃ swine manure biochar and biogas slurry（M₂S）treatment. WSS content did not change significantly in all treatments. HA and FA content were significantly increased by 0.57 g/kg and 70.0% in combined application of 2% 650 ℃ swine manure biochar and biogas slurry（H₂S）treatment，respectively，the HM content in M₂S treatment was significantly increased by 100.8%，and humus component content was dominated by HM； PQ value was significantly increased by 21.8% in H₂S treatment，laccase activity was decreased by 30.5% in combined application of 2% 350 ℃ swine manure biochar and biogas slurry（L₂S）treatment，and SPOD and SPPO activities were significantly increased in H₂S treatment by 30.1% and 35.0%，respectively.