To isolate and screen soybean rhizobia suitable for promoting growth，soybean symbiotic rhizobia were isolated and screened from the wild and major cultivated soybean varieties in moderate to severe saline-alkali soil of the Yellow River Delta agricultural hightech industrial demonstration zone，Dongying City，Shandong Province. Their nodulation and growth promoting functional characteristics were evaluated. A total of 84 pure cultures were isolated from the nodule samples of soybean，which belong to three genera and six species，and 96% of them belong to Ensifer. The results of functional testing showed that all of them can produce indole-3-acetic acid （IAA），32 strains could tolerate 2% NaCl，14 strains could produce iron carriers，7 strains could mineralize organic phosphorus，14 strains could dissolve inorganic phosphorus，and 6 strains could produce organophosphorus of over 200 mg/L. The results of hydroponic experiments showed that strains 63-2-1-1，306-2-1-1，and 63-2-19-1 could significantly alleviate the degree of salt damage to soybean seedlings. This study not only provided strain resources for the development of salt-tolerant microbial agents for soybeans in salinealkali soil but also had important theoretical and practical significance for the growth and yield increase of soybeans in saline-alkali soil.