To enhance the quality of measurement results for the determination of perfluoroalkyl substances（PFASs）in soil using liquid chromatography-triple quadrupole mass spectrometry（LC-TQMS），we analyzed and quantified the uncertainties associated with the measurement results in accordance with the relevant regulations governing the evaluation and expression of measurement uncertainties. The findings indicated that the relative standard uncertainties of the method ranged from 0.0701 to 0.4081，while the extended uncertainties varied between 16-268 ng·kg^-1（with an extended factor k=2）. Notably，the largest contribution to the uncertainty was attributed to standard curve fitting，which accounted for 38.3%- 82.6% of the total uncertainty. The second largest contribution arose from standard solution preparation，contributing between 8.35% and 36.9%，whereas the contribution from soil sample weighing was negligible.