The study aimed to investigate the effects of combined application of organic fertilizer and agricultural ferments on maize production，five treatments were conducted，including CK(no fertilizer)，YJ(organic fertilizer)，JS1(organic fertilizer+agricultural ferments spray at jointing stage)，JS2(organic fertilizer+agricultural ferments spray at filling stage)and JS3(organic fertilizer+agricultural ferments spray at both jointing stage and filling stage)，and their effects on soil physicochemical properties，nutrient content and yield of maize(Wugu 737)were compared. The results showed that:(1)Compared with CK，soil total nitrogen(N)，available phosphorus(P)，available potassium(K)and organic matter contents were increased under YJ treatment，and the addition of agricultural ferments enhanced the additive effect. JS3 treatment significantly improved the total N and available P content，while JS2 treatment improved the soil available K and organic matter content.(2)Compared with CK，JS3 treatment significantly improved N and P content in maize stem，but the K content was significantly reduced. The combination of organic fertilizer and agricultural ferments was beneficial to improve the N content of the stem. The combination of the organic fertilizer and agricultural ferments improved the total N and K content of leaves，and JS3 treatment showed an excellent improving effect. Combined application of organic fertilizer and agricultural ferments significantly increased N content and reduce K content of grains. Therefore，combination of organic fertilizer and agricultural ferments could improve the N uptake of maize tissues.(3)Grain yield increased by 44.71% and 58.84% under JS2 and JS3 treatments，respectively. In conclusion，the application of organic fertilizer+agricultural ferments at the jointing and filling period(JS3)could improve the physical and chemical properties of the soil and enhance the productivity of maize.