This study aimed to explore the effects of different green manures on tobacco production in central Yunnan，provide technical and theoretical support for improving the quality and efficiency of tobacco production in this region，and construct a reasonable green manure-tobacco rotation system.Based on the located field experiment started in 2017，the effects of different types of green manures on soil nutrients，yield，quality and economic benefits of tobacco in 2021—2022 were studied.Four treatments were set up in the fallow season of tobacco field，including winter fallow-tobacco，smooth vetch-tobacco，ryegrass-tobacco，and radish-tobacco rotation.Green manuring could improve soil properties，i.e.，the soil dissolved organic carbon and dissovloved organic nitrogen contents of all the three green manuring treatments significantly increased by 4.07-16.4 mg·kg-1 and 1.34-1.74 mg·kg-1，compared to the winter fallow (P<0.05).Moreover，soil organic carbon and inorganic nitrogen contents were on average 2.7 g·kg-1 and 13.5 mg·kg-1 (P<0.05) higher under smooth vetch treatment than in the winter fallow.Compared with winter fallow，the effective leaves average number of smooth vetch and radish treatment increased by 3.0 and 2.8 leaves pen /plant (P<0.05)，and the average annual increase of tobacco yield increased by 257 kg·hm-2.Generally，three green manuring treatments improved the ratio of potassium/chlorine in tobacco leaves，with increase in potassium concentration by 0.19% to 0.66% and decreased in chloride ion concentration by 0.14% to 0.18%，compared to the winter fallow.Additionally，radish significantly decreased the ratio of total sugar/nicotine in tobacco leaves.Compared with winter fallow，the average price and output value of tobacco in smooth vetch treatment increased by 2.2 Yuan·kg-1 and 13887 Yuan·hm-2，and the output value of ryegrass treatment increased by 9062 Yuan·hm-2，radish treatment increased by 3938 Yuan·hm-2 (P<0.05). The Mantel analysis found that tobacco yield was significantly correlated with mineral nitrogen and dissolved organic nitrogen. In summary，planting and incorporating green manure could improve the soil properties of the tobacco field，and then enhance the yield，quality and economic benefits of tobacco，and the smooth vetch showed the optimal performance.