In order to alleviate the problem of continuous crop barrier of pepper in karst mountainous area of Guizhou，six treatments were set up，including winter fallow-pepper rotation with no fertilizer application in the pepper season(CK)，winter fallow-pepper rotation with conventional fertilization during pepper season(PP)，oilseed rape-pepper rotation with 20% chemical fertilizer reduction in the pepper season(80%PY)，common vetch-pepper rotation with 20% chemical fertilizer reduction in the pepper season(80%PL)，oilseed rape-pepper rotation with 20% chemical fertilizer reduction and combined with biochar application in pepper season(80%PYB)，common vetch-pepper rotation and 20% chemical fertilizer reduction combined with biochar application in pepper season(80%PLB).The effects of green manure rotation with biochar on the production capacity and economic benefits of continuous cropping peppers in karst mountainous areas were investigated.The results showed that green manure rotation and green manure rotation combined with biochar application treatments increased the yield of dry pepper by 54.65-1134.14 kg/hm²，with an increase of 2.11%-51.78%，compared with PP treatment.At the same time，it significantly increased the content of reducing sugars(with an increase of 2.72%-64.65%)and Vc(with an increase of 5.12%-93.62%)，and reduced the nitrate content(with a decrease of 7.94%-29.33%).Meanwhile，green manure-pepper rotation and green manure-pepper rotation combined with biochar application treatments also significantly increased the accumulation of nitrogen，phosphorus and potassium nutrients in pepper plants，which was conducive to the improvement of fertilizer use efficiency.In addition，the production value and net profit of peppers in green manure-pepper rotation and green manure-pepper rotation combined with biochar application treatments were increased by 2.11%-51.78% and 3.93%-52.13%，respectively，compared with PP treatment.In summary，green manurepepper rotation and green manure-pepper rotation combined with biochar application could improve the yield and quality of continuous cropping peppers in the karst mountainous areas of Guizhou，and could be conducive to nutrient accumulation and fertilizer use efficiency，while being able to achieve an economic increase in the income of continuous pepper cultivation.Overall，80%PYB treatment had the optimal effect on yield increase，quality improvement，and fertilizer utilization and economic benefit enhancement of continuous cropping peppers，therefore，it is recommerded to adopt the oilseed ropepepper rotation combined with biochar application mode as the optimal technical strategy to alleviate the continuous crop barrier of peppers in karst mountainous areas under the condition of reducing fertilizer application by 20%.