To facilitate the transportation and storage of earthworm manure，how to rapidly reduce its water content while retaining its fertilizer and microbial content is a significant problem confronted by the earthworm composting industry.In this research，earthworm manure transformed from pure cow manure and cow manure + straw was selected as the study object，and drying experiments were carried out on earthworm manure at five environmental drying temperatures(25，35，45，55，65℃)to investigate the influences of different drying temperatures on the comprehensive quality of earthworm manure，such as nutrients，humification components，and microbial indicators.The findings demonstrated that temperature had no substantial effect on the pH of earthworm manure，but had a significant impact on the EC value.When the drying temperature was 35℃，the total microbial content of the two types of earthworm manure reached the peak，which were 9.92×106 and 1.11×107 N/mL，respectively.There was no significant difference in the phosphorus and potassium content of cow manure-earthworm manure and cow manure-straw-earthworm manure with the increasing of drying temperature.The total available nutrient content of cow manure-straw-earthworm manure dried at 35℃ was higher than that of other groups.The humification degree of earthworm manure was the best at 35℃，and the humic acid content decreased significantly when the temperature was higher than 35℃.The comprehensive evaluation results indicated that 35℃ was the optimal drying temperature for earthworm manure.The seven indicators of total fungus number，conductivity，alkaline nitrogen，total actinomyces number，humic acid，total bacteria number，and total nitrogen were significantly affected by temperature and should be given attention during the drying process.The results of this study have significant implications for the dry storage and quality control of earthworm manure.