The effects of different nitrogen sources and their combined application on decomposition and nutrient release of wheat straw were studied to provide theoretical basis for improving nutrient utilization of wheat straw. In this study，nylon net bag method was used to carry out indoor rot test，taking wheat straw as the research object，12 treatments were set up:no nitrogen fertilizer，single application of urea and potassium nitrate，single application of ammonium chloride，single application of cow dung，single application of sheep manure，urea combined with cow manure，urea combined with sheep manure，potassium nitrate combined with cow manure，potassium nitrate combined with sheep manure，ammonium chloride combined with cow manure，goat manure with ammonium chloride. The results showed that the decomposition of wheat straw and the release of carbon and potassium were fast in the early stage and slow in the late stage. In the whole culture process，with the extension of culture time，the decomposition rate of wheat straw increased gradually. Urea，cow dung，urea combined with sheep dung，potassium nitrate combined with cow dung and ammonium chloride combined with cow dung treatments promoted the degradation of wheat straw. Urea combined with sheep manure treatment had the best decomposing effect on wheat straw. According to the kinetic equation fitting，cow manure treatment had the best effect on promoting straw decomposition among the different manure fertilizers，and the decomposition rate constant reached 2.43×10-2 d-1. Urea combined with sheep manure treatment had the strongest effect on wheat straw，and the decomposition rate constant reached 4.90×10-2 d-1. At the end of the test，the wheat straw C/N was decreased to 33-68. The C/N of wheat straw treated with urea combined with sheep manure was the lowest，which was more conducive to straw decomposition. In the process of nutrient release，the application of potassium nitrate slowed down the nutrient release in straw，and the residual weight of carbon，nitrogen，phosphorus and potassium in wheat straw residues was 50.9%-65.6%，53.4%-91.6%，34.5%-60.2% and 5.31%-8.64% of its initial weight，respectively. The nutrient release rate was ranked as potassium > phosphorus > carbon> nitrogen. In conclusion，urea combined with sheep manure treatment had the best effect on straw decomposition. In terms of nutrient release，urea combined with sheep manure could promote the release of carbon and potassium from straw when organic and inorganic fertilizers were combined applied. Potassium nitrate combined with cow manure treatment promoted the release of nitrogen and phosphorus. In the case of adding organic nitrogen or inorganic nitrogen，the former was better than the latter in promoting the nutrient release of straw.