羊粪堆肥纤维素降解细菌的分离鉴定及产酶条件优化
作者:
作者单位:

(石河子大学动物科技学院,新疆 石河子 832000)

作者简介:

朱婷婷(1997-),硕士研究生,研究方向为基础兽医。E-mail:17852177632@163.com。

通讯作者:

基金项目:

基金项目:兵团财政科技计划项目—新疆生产建设兵团重点领域科技攻关计划项目(2021AB014)。


Isolation,identification and optimization of enzyme production conditions of cellulose-degrading bacteria from sheep manure compost
Author:
Affiliation:

(College of Animal Science and Technology,Shihezi University,Shihezi Xinjiang 832000)

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
    摘要:

    旨在挖掘具有纤维素降解能力的细菌菌株,丰富羊粪堆肥功能菌剂的菌种资源。根据羊粪堆肥过程中的温度变化将其分为不同阶段,采集各阶段样本,通过16S rRNA测序分析优势菌群;分离筛选具有纤维素降解能力的菌株,选择最优菌株进行菌种鉴定并优化产酶条件,进行堆肥验证试验,评价实际效果。结果显示:羊粪好氧堆肥过程可分为3个阶段,即初始阶段T1(第0 d)、高温期T2(1~12 d)、降温期T3(13~35 d)。在堆肥过程中有6个优势菌群(变形菌门、拟杆菌门、厚壁菌门、放线杆菌门、异常球菌和芽单孢菌门)的丰度呈现明显变化;相比于T1、T3期,T2期变形菌门和厚壁菌门的丰度明显升高。试验分离出30株具有纤维素降解能力的菌株,经检测11株菌降解能力较好,其中T2期的菌株G11 羧甲基纤维素(CMC)酶活性含量最高,鉴定为约翰逊不动杆菌(Acinetobacter johnsonii)。该菌株在37℃、接种量1%、初始pH 8、培养48 h时CMC酶活性达到(33.67±0.94)U/mL,较基础培养提高了22.4%,堆肥试验表明,在施加原有菌剂的基础上加入该菌株,纤维素酶活性峰值增高,达到94.49 U/g,漆酶活性同步增强。综上所述,筛选出的细菌G11具有较好的纤维素降解能力,可为羊粪堆肥菌剂的研发及应用提供理论依据。

    Abstract:

    The aimed to explore cellulose-degrading bacterial strains from sheep manure composting to enrich the microbial resources of functional inoculants for sheep manure composting. According to the temperature changes during the composting process of sheep manure,it was divided into different stages,and samples were collected from each stage. The dominant microbial community was analyzed by 16S rRNA sequencing. Strains with cellulose degradation ability were separated and screened. The optimal strain was selected for strain identification and the enzyme production conditions were optimized. The composting validation experiment was conducted,and the actual effects were evaluated. The results showed that the aerobic composting process of sheep manure was divided into three stages:initial phase(T1,Day 0),thermophilic phase(T2,Days 1-12) and cooling phase(T3,Days 13-35). 16S rRNA sequencing revealed significant shifts in the relative abundances of six dominant phyla(Proteobacteria,Bacteroidetes,Firmicutes,Actinobacteria,Deinococcus-Thermus and Gemmatimonadetes) across these stages. Notably,Proteobacteria and Firmicutes exhibited marked enrichment during the T2 phase compared to T1 and T3 phase. Thirty cellulose-degrading strains were isolated,with 11 demonstrating superior activity. Strain G11,isolated from T2 phase,identified as Acinetobacter john sonin showed the highest carboxymethylcellulase(CMC)activity(33.67±0.94)U/mL under optimized conditions(37℃,1% inoculum,pH 8,48 h cultivation),representing a 22.4% improvement over the basal medium. Compost validation trials confirmed that supplementing the existing inoculant with strain G11 increased peak cellulase activity to 94.49 U/g,and enhanced laccase activity synergistically. The results highlighted the potential of strain G11 as a high-efficiency cellulose-degrading agent for accelerating sheep manure composting. This study provided a theoretical basis for developing multifunctional microbial inoculants in agricultural waste management.

    参考文献
    相似文献
    引证文献
引用本文

朱婷婷,张栋梁,石荣华,刘磊,胡广东,曾维斌,王艳萍.羊粪堆肥纤维素降解细菌的分离鉴定及产酶条件优化[J].中国土壤与肥料123,2025,(9):202-210

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:2025-01-05
  • 最后修改日期:
  • 录用日期:2025-06-21
  • 在线发布日期: 2025-11-04
紧急通知:《中国土壤与肥料》编辑部没有给作者发“稿件深度复核邀请”的邮件,也没有“优秀论文深度复核计划”,请收到类似邮件的作者不要加微信,以免受骗。
关闭