解淀粉芽孢杆菌和矿物质添加对鸡粪堆肥腐殖质、木质纤维素和细菌多样性的影响
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(1.北京市农林科学院植物营养与资源环境研究所,北京 100097;2.东北农业大学资源与环境学院,黑龙江 哈尔滨 150030;3.北京市缓控释肥料工程技术研究中心,北京 100097)

作者简介:

蔡连凤(1999-),硕士研究生,研究方向为农业资源化利用。E-mail:1395974214@qq.com。

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基金项目:国家重点研发计划项目(2022YFD1601100);北京市


Effects of Bacillus amyloliquefaciens and mineral addition on humus,lignocellulose and bacterial diversity in chicken manure composting
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(1.Institute of Plant Nutrition and Resource,Beijing Academy of Agricultural and Forestry Science,Beijing 100097;2.College of Resources and Environment,Northeast Agricultural University,Harbin Heilongjiang 150030;3.Beijing Engineering Technology Research Center for Slow/Controlled-Release Fertilizer,Beijing 100097)

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    摘要:

    为探究解淀粉芽孢杆菌和不同矿物质添加对堆肥过程中木质纤维素降解、腐殖质形成和微生物多样性的影响。以鸡粪和木屑为原料,设置5个处理,分别为对照(CK)、添加基于物料干重2%解淀粉芽孢杆菌(T1)、2%解淀粉芽孢杆菌+10%膨润土(T2)、2%解淀粉芽孢杆菌+10%麦饭石(T3)和2%解淀粉芽孢杆菌+10%海泡石(T4),通过监测温度、木质纤维素、腐植酸和细菌多样性等指标,分析解淀粉芽孢杆菌和矿物质对堆肥过程的影响。结果表明,T2、T3和T4处理半纤维素显著降低12.63%~18.44%(P<0.05),纤维素含量显著降低10.48%~12.59%(P<0.05),其中T3处理效果最好(半纤维素和纤维素含量分别降低18.44%和12.59%);T2、T3、T4处理腐植酸和胡敏酸含量分别显著上升4.94%~6.17%(P<0.05)和11.54%~15.38%(P<0.05),其中T3处理效果较好(腐植酸和胡敏酸含量分别上升6.17%和15.38%)。与CK相比,T2、T3和T4处理胡敏酸与富里酸比值(HA/FA)分别显著升高16.91%、23.54%和12.77%(P<0.05),此外,所有处理的HA/FA在第14 d后均高于1.6,表明腐熟过程得到有效促进,且在第14 d后种子发芽指数均大于80%,达到腐熟标准。细菌群落结构在堆肥过程中发生了较大变化,各处理的细菌多样性均出现了明显提高,厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)、放线菌门(Actinobacteriota)和拟杆菌门(Bacteroidota)成为堆肥的优势菌群,拟杆菌门均呈现升高趋势,在T3处理中相对丰度较高(13.01%)。研究发现,解淀粉芽孢杆菌和矿物质的协同作用能促进纤维素和半纤维素降解,加速腐殖质的形成,并将腐熟时间缩短至14 d以内,其中解淀粉芽孢杆菌和麦饭石的协同作用在实际堆肥应用中具有较大潜力。

    Abstract:

    The research aimed to investigate the effects of Bacillus amyloliquefaciens and different mineral additions on lignocellulose degradation,humus formation and microbial diversity during composting. In this study,chicken manure and wood chips were used as raw materials,and five treatments were set up,including the control group (CK),with the addition of 2% Bacillus amyloliquefaciens based on the dry weight of the material (T1),2% Bacillus amyloliquefaciens+10% bentonite clay (T2),2% Bacillus amyloliquefaciens+10% mackerel (T3),and 2% Bacillus amyloliquefaciens+10% sea foam (T4). The effect of the different minerals added on the degradation of lignocellulose and the formation of humus and microbial diversity during the composting process were analyzed through the monitoring of temperature,lignocellulose,humic acid,and bacterial diversity and other indicators to analyze the effects of Bacillus amyloliquefaciens and minerals on the composting process. The results showed that the hemicellulose of T2,T3 and T4 treatments was significantly reduced by 12.63%-18.44% (P<0.05) and thecellulose content was significantly reduced by 10.48%-12.59% (P<0.05),among which the best effect was achieved by the T3 treatment (hemicellulose content was reduced by 18.44% and cellulose content was reduced by 12.59%). The humic acid content of T2,T3 and T4 treatments was significantly increased (4.94%-6.17%,P < 0.05) and humic acid content was increased significantly (11.54%-15.38%,P < 0.05),among which the T3 treatment was more effective (humic acid increased by 6.17% and humic acid increased by 15.38%). Compared with CK,the humic acid to fulvic acid ratio of T2,T3 and T4 treatments was significantly increased by 16.91%,23.54% and 12.77% (P < 0.05),respectively. In addition,the humic acid to fulvic acid ratio of all treatments was higher than 1.6 after the 14th d,indicating that the humification process had been effectively promoted,and the seed germination indices were greater than 80% after 14 d,which reached the standard of decomposition. The bacterial community structure changed greatly during the composting process,and the bacterial diversity of all treatments was increased significantly,with Firmicutes,Proteobacteria,Actinobacteriota and Bacteroidota becoming the dominant bacterial groups in composting,and Bacteroidota showing an increasing trend in the composting process,with the Bacteroidota showing an increasing trend in the composting process,with higher relative abundance(13.01%)in T3 treatment. In this study,it was found that the synergistic effect of Bacillus amyloliquefaciens and Bacillus cereus could promote cellulose and hemicellulose degradation,accelerate humus formation,and shorten the decomposition time to less than 14 d. The synergistic effect of Bacillus amyloliquefaciens and Bacillus cereus had a greater potential for practical composting applications.

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蔡连凤,王学霞,丁建莉,薛文涛,刘建斌,魏丹,梁丽娜.解淀粉芽孢杆菌和矿物质添加对鸡粪堆肥腐殖质、木质纤维素和细菌多样性的影响[J].中国土壤与肥料123,2026,(3):192-204

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  • 收稿日期:2025-05-29
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  • 录用日期:2025-07-02
  • 在线发布日期: 2026-05-11
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