The effects of combined inoculation of Rhizobium and PGPR(M9Z，M10) on three active components in licorice were investigated. Licorice was used as the experimental material. The effects of single inoculation with Rhizobium or PGPR and their combined application were compared，using non-inoculated plants as the control (CK). The contents of crude protein，crude fat，total sugar，total saponin，and flavonoids were determined using the Kjeldahl method，Soxhlet extraction，phenol-sulfuric acid method，ultraviolet-visible spectrophotometry and spectrophotometry，respectively. The results demonstrated that combined inoculation significantly enhanced the accumulation of crude protein，crude fat，total sugar，total saponin，and flavonoids (P<0.05)，Compared with CK，the combined administration of Glycyrrhiza uralensis was increased by 49.57% and 46.84%，21.02% and 31.29%，21.68% and 23.42%，25.10% and 25.08%，15.87% and 22.06%，respectively；the combined administration of Glycyrrhiza glabra was increased by 28.14% and 26.38%，25.9% and 31.24%，16.41% and 21.14%，23.50% and 36.28%，16.20% and 19.14%，respectively；the combined administration of Glycyrrhiza inflata increased by 37.27% and 40.33%，24.12% and 18.11%，21.18% and 22.48%，34.68% and 31.96%，21.64% and 23.84%，respectively. Furthermore，these five components exhibited significant correlations with one another (P<0.05). Combined inoculation of Rhizobium and PGPR could effectively promote the accumulation of active components in licorice，providing a theoretical foundation for further elucidating the mechanisms underlying the enhancement of effective components in licorice through combined inoculation.