Phosphorus is one of the major elements in soil and is indispensable for life activities.Phosphorus-solubilizing microorganisms play a very important role in the transformation of phosphorus in the soil.Currently，studies on phosphorussolubilizing fungi mainly focus on Aspergillus，Penicillium，Fusarium，Trichoderma，etc.，yet few studies focused on the phosphorus-solubilizing ability of Basidiomycetes.Therefore，this study used Trametes gibbosa SICAU-T41 as the research material，and applied the solid fermented product of strain SICAU-T41 on flue-cured tobacco pot cultivation experiments，chemical analysis，quantitative real-time polymerase chain reaction，and high-throughput sequencing were combined to analyze the rhizosphere microecosystem effects of strain on flue-cured tobacco.The results showed that the solid fermented product significantly promoted the growth and development of flue-cured tobacco.In the pot experiment，the treatmen(t T4) with the application of the solid fermented product of SICAU-T41 had significantly increased the plant height，root length，maximum leaf length，maximum leaf width，fresh weight，and stem diameter of flue-cured tobacco，which were 133.87%，27.48%，75.17%，105.45%，299.04%，and 42.04% higher than the control treatment，respectively.The contents of soil organic matter，alkali-hydrolyzed nitrogen，available phosphorus，water-soluble phosphorus and phosphorus extracted by sodium bicarbonate increased significantly( P<0.05).The qPCR quantitative results showed that the colonization amount of the strain in the T4 treatment reached 3.38×107 copies/g，which was increased by 112% compared with that of the liquid microbial inoculum treatmen(t 1.60×107 copies/g).The dominant bacterial phyla in the soil were Proteobacteria，Acidobacteriota，and Actinobacteriota，while the dominant fungal phyla were Ascomycota，Mortierellomycota，and Basidiomycota.The relative abundances of Mucilaginibacter，Ochrobactrum，and Sphingobacterium，and Candida，and Nectria were significantly increased.The solid-state fermentation product of strain SICAU-T41 enhanced the fluecured tobacco agrcnomic performance，increased the soil nutrients contents，and varied the soil microbial communities，and showed prospective application value.