In this study，blueberry cultivars‘ Blue gold’ and‘ Spartan’ were used as test materials，and amino acid selenium foliar fertilizer( 600-fold dilution) was sprayed during the flowering and fruit-setting period.The aim was to explore the effects of foliar spraying of amino acid selenium on leaf quality，fruit quality，and fruit selenium content of blueberries，so as to provide a theoretical basis for the production of selenium-enriched blueberries.The results showed that compared with the control of spraying water，spraying amino acid selenium significantly improved leaf quality: the leaf chlorophyll content and leaf thickness of‘ Blue Gold’ and‘ Spartan’ increased by 39.13%，22.33% and 14.37%，13.90%，respectively.Meanwhile，the nutritional value of fruits was significantly enhanced，with vitamin C and selenium contents increasing significantly.For‘ Blue Gold’，the increases were 11.43%( vitamin C)and 1866.67%( selenium)； for‘ Spartan’，the increases were 27.45%( vitamin C) and 2577.78%( selenium).There were differences in the response of different varieties to amino acid selenium foliar fertilizer:‘ Blue gold’ performed better in improving leaf quality such as chlorophyll content and the thickness of upper and lower leaf epidermis，while‘Spartan’ stood out in increasing fruit selenium content and comprehensive fruit quality.In this study，the fruit selenium accumulation capacity of‘ Spartan’ was stronger than that of‘ Blue Gold’，which provided a theoretical basis for the rational application of amino acid selenium foliar fertilizer in high-quality and efficient blueberry production.