The study aimed to establish an extraction and detection method for N-(n-butyl)thiophosphoric triamide(NBPT) in different types of soil.Separation and quantitative analysis of NBPT were performed on a Pursuit XRs 5 C18 column( 150 mm×4.6 mm) using a mobile phase of (acetonitrile)∶V(water) = 1∶4 at a flow rate of 1.0 mL/min.The soil extract solution( pH 9.18 buffer) was analyzed at a detection wavelength of 210 nm.The determination coefficient was 0.9999.The standard deviation ranged from 0.05 to 1.61 mg/L across soil types，with a coefficient of variation of 0.24%-4.22%.The spiked recovery rates were between 90.44% and 98.64%.This method is rapid and sensitive，making it suitable for the extraction and determination of NBPT in various soil types.